More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| APEX nuclease(apurinic/apyrimidinic endonuclease) 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human APEX2(504-518aa DPSSRCNFFLWSRPS), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | APEX2 |
Protein | DNA-(apurinic or apyrimidinic site) lyase 2 |
Uniprot ID | Q9UBZ4 |
Function | Function as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes. |
Tissue Specificity | Highly expressed in brain and kidney. Weakly expressed in the fetal brain. |
Sub-cellular localization | Nucleus. Cytoplasm. Mitochondrion . Note: Together with PCNA, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. |
Sequence Similarities | Belongs to the DNA repair enzymes AP/ExoA family. |
Aliases | AP endonuclease 2 antibody|AP endonuclease XTH2 antibody|APE 2 antibody|APE2 antibody|APEX 2 antibody|APEX L2 antibody|APEX nuclease(apurinic/apyrimidinic endonuclease) 2 antibody|APEX nuclease 2 antibody|APEX nuclease like 2 antibody|APEX nuclease-like 2 antibody|APEX2 antibody|APEX2_HUMAN antibody|APEXL 2 antibody|APEXL2 antibody|Apurinic apyrimidinic endonuclease 2 antibody|Apurinic-apyrimidinic endonuclease 2 antibody|Apurinic/apyrimidinic endonuclease 2 antibody|Apurinic/apyrimidinic endonuclease like 2 antibody|C430040P13Rik antibody|DNA(apurinic or apyrimidinic site) lyase 2 antibody|DNA apurinic or apyrimidinic site lyase 2 antibody|DNA-(apurinic or apyrimidinic site) lyase 2 antibody|EC 4.2.99.18 antibody|OTTHUMP00000023390 antibody|OTTHUMP00000061908 antibody|XTH 2 antibody|XTH2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- APEX2 antibody, ASA-B0112, Western blotting
Anti APEX2 (ASA-B0112) at 0.5ug/ml
SMMC Whole Cell Lysate at 40ug
Predicted band size: 57KD
Observed band size: 57KD
Anti APEX2 (ASA-B0112) at 0.5ug/ml
SMMC Whole Cell Lysate at 40ug
Predicted band size: 57KD
Observed band size: 57KD
Anti- APEX2 antibody, ASA-B0112, IHC(P)
Human Mammary Cancer Tissue
Human Mammary Cancer Tissue