More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| APEX nuclease (multifunctional DNA repair enzyme) 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human APE1 recombinant protein (Position: P2-L318). Human APE1 shares 94% and 93% amino acid (aa) sequences identity with mouse and rat APE1, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | APEX1 |
Protein | APEX nuclease (Multifunctional DNA repair enzyme) 1 |
Uniprot ID | Q5TZP7 |
Function | Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'- phosphate (dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination (CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression. Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Associates, together with YBX1, on the MDR1 promoter. Together with NPM1, associates with rRNA. Binds DNA and RNA. |
Tissue Specificity | |
Sub-cellular localization | Nucleus. Nucleus, nucleolus. Nucleus speckle. Endoplasmic reticulum. Cytoplasm. Note: Detected in the cytoplasm of B-cells stimulated to switch (By similarity). Colocalized with SIRT1 in the nucleus. Colocalized with YBX1 in nuclear speckles after genotoxic stress. Together with OGG1 is recruited to nuclear speckles in UVA-irradiated cells. Colocalized with nucleolin and NPM1 in the nucleolus. Its nucleolar localization is cell cycle dependent and requires active rRNA transcription. Colocalized with calreticulin in the endoplasmic reticulum. Translocation from the nucleus to the cytoplasm is stimulated in presence of nitric oxide (NO) and function in a CRM1-dependent manner, possibly as a consequence of demasking a nuclear export signal (amino acid position 64-80). S-nitrosylation at Cys-93 and Cys-310 regulates its nuclear-cytosolic shuttling. Ubiquitinated form is localized predominantly in the cytoplasm. |
Sequence Similarities | Belongs to the DNA repair enzymes AP/ExoA family. |
Aliases | AP endonuclease 1 antibody|AP endonuclease class I antibody|AP lyase antibody|APE 1 antibody|APE antibody|APE-1 antibody|APEN antibody|APEX 1 antibody|APEX antibody|APEX nuclease (multifunctional DNA repair enzyme) 1 antibody|Apex nuclease 1 antibody|Apex nuclease antibody|APEX1 antibody|APEX1_HUMAN antibody|Apurinic endonuclease antibody|Apurinic-apyrimidinic endonuclease 1 antibody|Apurinic/apyrimidinic (abasic) endonuclease antibody|Apurinic/apyrimidinic endonuclease 1 antibody|Apurinic/apyrimidinic exonuclease antibody|APX antibody|BAP1 antibody|Deoxyribonuclease (apurinic or apyrimidinic) antibody|DNA (apurinic or apyrimidinic site) lyase antibody|DNA-(apurinic or apyrimidinic site) lyase, mitochondrial antibody|EC 4.2.99.18 antibody|HAP 1 antibody|HAP1 antibody|Human Apurinic endonuclease 1 antibody|MGC139790 antibody|Multifunctional DNA repair enzyme antibody|Redox factor 1 antibody|Redox factor-1 antibody|REF 1 antibody|REF 1 protein antibody|REF-1 antibody|REF1 antibody|REF1 protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-APEX1 antibody, ASA-B0110, Western blot
All lanes: Anti APEX1 (ASA-B0110) at 0.5ug/ml
Lane 1: NRK Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: PC-12 Whole Cell Lysate at 40ug
Lane 4: RH35 Whole Cell Lysate at 40ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Lane 6: MCF Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Lane 8: Human Placenta Tissue Lysate at 50ug
Lane 9: A431 Whole Cell Lysate at 40ug
Predicted band size: 39KD
Observed band size: 39KD
All lanes: Anti APEX1 (ASA-B0110) at 0.5ug/ml
Lane 1: NRK Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: PC-12 Whole Cell Lysate at 40ug
Lane 4: RH35 Whole Cell Lysate at 40ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Lane 6: MCF Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Lane 8: Human Placenta Tissue Lysate at 50ug
Lane 9: A431 Whole Cell Lysate at 40ug
Predicted band size: 39KD
Observed band size: 39KD
Anti-APEX1 antibody, ASA-B0110, IHC(P)
Human Lung Cancer Tissue