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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| annexin A5 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Annexin V(88-102aa SRLYDAYELKHALKG), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ANXA5 |
Protein | Annexin A5 |
Uniprot ID | P08758 |
Function | This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | Belongs to the annexin family. |
Aliases | Anchorin CII antibody|Annexin 5 antibody|Annexin A5 antibody|Annexin V antibody|Annexin-5 antibody|Annexin5 antibody|AnnexinA5 antibody|AnnexinV antibody|ANX 5 antibody|ANX A5 antibody|ANX5 antibody|ANXA5 antibody|ANXA5_HUMAN antibody|Calphobindin I antibody|CBP I antibody|CBP-I antibody|Endonexin II antibody|ENX 2 antibody|ENX2 antibody|Lipocortin V antibody|PAP I antibody|PAP-I antibody|Placental anticoagulant protein 4 antibody|Placental anticoagulant protein I antibody|PLACENTAL PROTEIN 4 antibody|PP 4 antibody|PP4 antibody|Thromboplastin inhibitor antibody|VAC alpha antibody|VAC-alpha antibody|Vascular anticoagulant alpha antibody|Vascular anticoagulant-alpha antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Annexin V antibody, ASA-B0099, Western blotting
All lanes: Anti Annexin V (ASA-B0099) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 3: Rat Ovary Tissue Lysate at 50ug
Lane 4: Rat Lung Tissue Lysate at 50ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Lane 8: JURKAT Whole Cell Lysate at 40ug
Lane 9: SGC Whole Cell Lysate
All lanes: Anti Annexin V (ASA-B0099) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 3: Rat Ovary Tissue Lysate at 50ug
Lane 4: Rat Lung Tissue Lysate at 50ug
Lane 5: MCF-7 Whole Cell Lysate at 40ug
Lane 6: SMMC Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Lane 8: JURKAT Whole Cell Lysate at 40ug
Lane 9: SGC Whole Cell Lysate
Anti-Annexin V antibody, ASA-B0099, IHC(P)
Rat Liver Tissue
Rat Liver Tissue
Anti-Annexin V antibody, ASA-B0099, ICC
HCT116 Cell
HCT116 Cell