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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| annexin A3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of rat Annexin A3(124-141aa RQMKEISQAYYTAYKKNL), different from the related human and mouse sequences by two amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ANXA3 |
Protein | Annexin A3 |
Uniprot ID | P14669 |
Function | Inhibitor of phospholipase A2, also possesses anti- coagulant properties. |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | Belongs to the annexin family. |
Aliases | 2-cyclic phosphate 2-phosphohydrolase antibody|35-alpha calcimedin antibody|Annexin A3 antibody|Annexin III antibody|Annexin-3 antibody|ANX3 antibody|ANXA 3 antibody|Anxa3 antibody|ANXA3_HUMAN antibody|Inositol 1 2 cyclic phosphate 2 phosphohydrolase antibody|Inositol 1 antibody|Lipocortin III antibody|OTTHUMP00000160701 antibody|OTTHUMP00000219237 antibody|OTTHUMP00000219238 antibody|PAP III antibody|PAP-III antibody|Placental anticoagulant protein III antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Annexin A3 antibody, ASA-B0096, Western blotting
All lanes: Anti Annexin A3 (ASA-B0096) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Testis Tissue Lysate at 50ug
Lane 3: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 4: Rat Lung Tissue Lysate at 50ug
Lane 5: SW620 Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: SMMC Whole Cell Lysate at 40ug
Lane 8: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 36KD
All lanes: Anti Annexin A3 (ASA-B0096) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Testis Tissue Lysate at 50ug
Lane 3: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 4: Rat Lung Tissue Lysate at 50ug
Lane 5: SW620 Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: SMMC Whole Cell Lysate at 40ug
Lane 8: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Anti-Annexin A3 antibody, ASA-B0096, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue