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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| annexin A2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human Annexin A2(121-141aa RAEDGSVIDYELIDQDARDLY), different from the rat sequence by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ANXA2 |
Protein | Annexin A2 |
Uniprot ID | P07355 |
Function | Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9 (PubMed:18799458, PubMed:24808179, PubMed:22848640). |
Tissue Specificity | |
Sub-cellular localization | Secreted, extracellular space, extracellular matrix, basement membrane. |
Sequence Similarities | Belongs to the annexin family. |
Aliases | Annexin A2 antibody|Annexin II antibody|Annexin II, heavy chain antibody|Annexin-2 antibody|ANX 2 antibody|ANX2 antibody|ANX2L4 antibody|ANXA2 antibody|ANXA2_HUMAN antibody|arylsulfatase B antibody|CAL1H antibody|Calpactin I heavy chain antibody|calpactin I heavy polypeptide(p36) antibody|Calpactin-1 heavy chain antibody|chromobindin 8 antibody|Chromobindin-8 antibody|LIP2 antibody|Lipocortin II antibody|LPC2 antibody|LPC2D antibody|P36 antibody|P36 protein antibody|PAP-IV antibody|placental anticoagulant protein IV antibody|Protein I antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Annexin A2 antibody, ASA-B0095, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Rat Ovary Tissue Lysate
Lane 4: MCF-7 Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: A549 Cell Lysate
Lane 7: JURKAT Cell Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Rat Ovary Tissue Lysate
Lane 4: MCF-7 Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: A549 Cell Lysate
Lane 7: JURKAT Cell Lysate
Anti-Annexin A2 antibody, ASA-B0095, IHC(P)
Human Intestinal Cancer Tissue
Human Intestinal Cancer Tissue