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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| annexin A1 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Annexin A1(3-24aa MVSEFLKQAWFIENEEQEYVQT). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ANXA1 |
Protein | Annexin A1 |
Uniprot ID | P04083 |
Function | Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. |
Tissue Specificity | |
Sub-cellular localization | Nucleus . Cytoplasm . Cell projection, cilium . Basolateral cell membrane . Note: Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium. Found in the cytoplasm of type II pneumocytes and alveolar macrophages. |
Sequence Similarities | Belongs to the annexin family. |
Aliases | Annexin 1 antibody|Annexin A1 antibody|Annexin I(lipocortin I) antibody|Annexin I antibody|Annexin-1 antibody|AnnexinA1 antibody|AnnexinI antibody|ANX 1 antibody|ANX A1 antibody|ANX1 antibody|ANXA 1 antibody|ANXA1 antibody|ANXA1 protein antibody|ANXA1_HUMAN antibody|Calpactin 2 antibody|Calpactin II antibody|Calpactin-2 antibody|CalpactinII antibody|Chromobindin 9 antibody|Chromobindin-9 antibody|Chromobindin9 antibody|HGNC:533 antibody|Lipocortin 1 antibody|Lipocortin I antibody|Lipocortin1 antibody|LipocortinI antibody|LPC 1 antibody|LPC1 antibody|p35 antibody|Phospholipase A2 inhibitory protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Annexin A1 antibody, ASA-B0092, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: PANC Cell Lysate
Lane 4: COLO320 Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 1: U87 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: PANC Cell Lysate
Lane 4: COLO320 Cell Lysate
Lane 5: SMMC Cell Lysate
Anti-Annexin A1 antibody, ASA-B0092, IHC(P)
Human Placenta Tissue
Human Placenta Tissue
Anti-Annexin A1 antibody, ASA-B0092, IHC(P)
Human Tonsil Tissue
Human Tonsil Tissue