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Annexin A1 Antibody

ASA-B0093

$365.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

annexin A1PolyclonalIgGRabbitHuman, Mouse, RatIHC-P, IHC-F, WBImmunogen affinity purified.

Immunogen

E.coli-derived human Annexin A1 recombinant protein (Position: A2-N346). Human Annexin A1 shares 88% and 89% amino acid (aa) sequences identity with mouse and rat Annexin A1, respectively.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

ANXA1

Protein

Annexin A1

Uniprot ID

P04083

Function

Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.

Tissue Specificity

 

Sub-cellular localization

Nucleus . Cytoplasm . Cell projection, cilium . Basolateral cell membrane . Note: Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium. Found in the cytoplasm of type II pneumocytes and alveolar macrophages.

Sequence Similarities

Belongs to the annexin family.

Aliases

Annexin 1 antibody|Annexin A1 antibody|Annexin I (lipocortin I) antibody|Annexin I antibody|Annexin-1 antibody|AnnexinA1 antibody|AnnexinI antibody|ANX 1 antibody|ANX A1 antibody|ANX1 antibody|ANXA 1 antibody|ANXA1 antibody|ANXA1 protein antibody|ANXA1_HUMAN antibody|Calpactin 2 antibody|Calpactin II antibody|Calpactin-2 antibody|CalpactinII antibody|Chromobindin 9 antibody|Chromobindin-9 antibody|Chromobindin9 antibody|Lipocortin 1 antibody|Lipocortin I antibody|Lipocortin1 antibody|LipocortinI antibody|LPC 1 antibody|LPC1 antibody|p35 antibody|Phospholipase A2 inhibitory protein antibody

Application Details

ApplicationConcentration*SpeciesValidated Using**
Western blot0.1-0.5μg/mlHumanAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)0.5-1μg/mlHuman, RatAssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section)0.5-1μg/mlMouseAssaySolutio's IHC/ICC Detection kit


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information

Anti-Annexin A1recombinant
 
Anti-Annexin A1 antibody, ASA-B0093, Western blot
All lanes: Anti AnnexinA1 (ASA-B0093) at 0.5ug/ml
WB: Recombinant Human AnnexinA1 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti-Annexin A1natural
 
Anti-Annexin A1 antibody, ASA-B0093, Western blot
All lanes: Anti AnnexinA1 (ASA-B0093) at 0.5ug/ml
Lane 1: HEPG2 Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: PANC Whole Cell Lysate at 40ug
Lane 5: SMMC Whole Cell Lysate at 40ug
Lane 6: SW620 Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: A549 Whole Cell Lysate at 40ug
Lane 9: JURKAT Whole Cell Lysate at 40ug
 
 
 
 
 Anti-Annexin A1hm
 
Anti-Annexin A1 antibody, ASA-B0093, IHC(P)
Human Tonsil Tissue
 
 
 
 
Anti-Annexin A1rat
 
Anti-Annexin A1 antibody, ASA-B0093, IHC(P)
Rat Lung Tissue
 
 

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