More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| androgen receptor | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Androgen Receptor(822-838aa LKNQKFFDELRMNYIKE), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | AR |
Protein | Androgen receptor |
Uniprot ID | P10275 |
Function | Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. Transcription activation is down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3. |
Tissue Specificity | Isoform 2 is mainly expressed in heart and skeletal muscle. |
Sub-cellular localization | Nucleus. Cytoplasm. Note: Predominantly cytoplasmic in unligated form but translocates to the nucleus upon ligand-binding. Can also translocate to the nucleus in unligated form in the presence of GNB2L1. |
Sequence Similarities | Belongs to the nuclear hormone receptor family. NR3 subfamily. |
Aliases | AIS antibody|ANDR_HUMAN antibody|Androgen receptor(dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) antibody|Androgen receptor antibody|AR antibody|DHTR antibody|Dihydro Testosterone Receptor antibody|Dihydrotestosterone receptor antibody|HUMARA antibody|HYSP1 antibody|KD antibody|NR3C4 antibody|Nuclear receptor subfamily 3 group C member 4 antibody|SBMA antibody|SMAX1 antibody|Spinal and bulbar muscular atrophy antibody|TFM antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Androgen Receptor antibody, ASA-B0081, Western blotting
Rat Testis Tissue Lysate
Rat Testis Tissue Lysate
Anti-Androgen Receptor antibody, ASA-B0081, IHC(P)
Human Prostatic Cancer Tissue
Human Prostatic Cancer Tissue
Anti-Androgen Receptor antibody, ASA-B0081, Western blotting
22RV1 Cell Lysate
22RV1 Cell Lysate