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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| adenosylmethionine decarboxylase 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human AMD1(315-330aa FNDYNFVFTSFAKKQQ), identical to the related mouse and rat sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | AMD1 |
Protein | S-adenosylmethionine decarboxylase proenzyme(AdoMetDC/SAMDC) |
Uniprot ID | P17707 |
Function | Essential for biosynthesis of the polyamines spermidine and spermine. Promotes maintenance and self-renewal of embryonic stem cells, by maintaining spermine levels (By similarity). |
Tissue Specificity | |
Sub-cellular localization | |
Sequence Similarities | |
Aliases | Adenosylmethionine decarboxylase 1 antibody|AdoMetDC antibody|AMD1 antibody|DCAM_HUMAN antibody|EC 4.1.1.50 antibody|FLJ26964 antibody|S adenosylmethionine decarboxylase 1 antibody|S adenosylmethionine decarboxylase proenzyme antibody|S-adenosylmethionine decarboxylase beta chain antibody|SAMDC antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-AMD1 antibody, ASA-B0079, IHC(P)
Human Mammary Cancer Tissue
Human Mammary Cancer Tissue
Anti-AMD1 antibody, ASA-B0079, Western blotting
All lanes: Anti AMD1 (ASA-B0079) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted band size: 38KD
Observed band size: 38KD
All lanes: Anti AMD1 (ASA-B0079) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted band size: 38KD
Observed band size: 38KD