More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| actin, alpha 2, smooth muscle, aorta | Monoclonal | IgG | Mouse | Human, Mouse, Rat | IHC-P, IHC-F, WB | Ascites |
Immunogen |
| N-terminal synthetic decapeptide of alpha-smooth muscle actin. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | 1867 |
Protein | Actin, aortic smooth muscle |
Uniprot ID | Human(P62736), Mouse(P62737), Rat(P62738) |
Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
Tissue Specificity | |
Sub-cellular localization | Cytoplasm, cytoskeleton. |
Sequence Similarities | |
Aliases | AAT6|ACTSA|MYMY5|ACTSA|alpha-cardiac actin|cell growth-inhibiting gene 46 protein |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 2μg/ml | Human, mouse, rat. | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.1-0.5μg/ml | Human, mouse, rat. | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.1-0.5μg/ml | Human, mouse, rat. | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-alpha-Smooth Muscle Actin antibody (monoclonal), ASA-B0077, IHC(P)
Rat Cardiac Muscle Tissue
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