More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| actinin, alpha 2 | Monoclonal | IgG | Mouse | Human, Mouse, Rabbit, Rat | IHC-P, IHC-F, WB | Ascites |
Immunogen |
| Rabbit skeletal alpha-actinin. |
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ACTN2 |
Protein | Alpha-actinin-2 |
Uniprot ID | Human(P35609), Mouse(Q9JI91), Rat(D3ZCV0) |
Function | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. |
Tissue Specificity | Expressed in both skeletal and cardiac muscle. |
Sub-cellular localization | Cytoplasm, myofibril, sarcomere, Z line. |
Sequence Similarities | Belongs to the alpha-actinin family. |
Aliases | CMD1AA|Alpha-actinin skeletal muscle isoform 2|F-actin cross-linking protein |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 3.5μg/ml | Human, mouse, rat, rabbit. | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 3.5-7μg/ml | Human, mouse, rat, rabbit. | AssaySolutio's IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 3.5-7μg/ml | Human, mouse, rat, rabbit. | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
