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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| lactalbumin, alpha- | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human alpha Lactalbumin(20-37aa KQFTKCELSQLLKDIDGY). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | LALBA |
Protein | Alpha-lactalbumin |
Uniprot ID | P00709 |
Function | Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins. |
Tissue Specificity | Mammary gland specific. Secreted in milk. |
Sub-cellular localization | Secreted. |
Sequence Similarities | |
Aliases | a-LACTA antibody|alfaLA antibody|Alpha-lactalbumin antibody|Lactalbumin alpha antibody|Lactose synthase B protein antibody|LALBA antibody|LALBA_HUMAN antibody|Lysozyme-like protein 7 antibody|LYZL7 antibody|MGC138521 antibody|MGC138523 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-alpha Lactalbumin antibody, ASA-B0076, Western blotting
All lanes: Anti alpha Lactalbumin (ASA-B0076) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: MM231 Whole Cell Lysate at 40ug
Lane 3: MM453 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted band size: 16KD
Observed band size: 25KD
All lanes: Anti alpha Lactalbumin (ASA-B0076) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: MM231 Whole Cell Lysate at 40ug
Lane 3: MM453 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted band size: 16KD
Observed band size: 25KD
Anti-alpha Lactalbumin antibody, ASA-B0076, IHC(P)
Human Mammary Cancer Tissue
Human Mammary Cancer Tissue