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More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| serpin peptidase inhibitor, clade A(alpha-1 antiproteinase, antitrypsin), member 3 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human alpha 1 Antichymotrypsin (218-232aa EMPFDPQDTHQSRFY). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | SERPINA3 |
Protein | Alpha-1-antichymotrypsin |
Uniprot ID | P01011 |
Function | Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. |
Tissue Specificity | Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Belongs to the serpin family. |
Aliases | SERPINA3 antibody|AACT antibody|AACT_HUMAN antibody|ACT antibody|Alpha 1 Antichymotrypsin 1 antibody|Alpha 1 Antichymotrypsin 2 antibody|Alpha 1 Antichymotrypsin 3 antibody|Alpha 1 Antitrypsin Deficiency antibody|Alpha-1-antichymotrypsin His-Pro-less antibody|Anti Elastase antibody|Antichymotrypsin antibody|Cell growth inhibiting gene 24/25 protein antibody|Cell growth-inhibiting gene 24/25 protein antibody|Clade A(Alpha 1 Antiproteinase Antitrypsin) antibody|GIG24 antibody|GIG25 antibody|Growth inhibiting protein 24 antibody|Growth inhibiting protein 25 antibody|MGC88254 antibody|PI1 antibody|Protease Inhibitor 1 antibody|Serine(or Cysteine) Proteinase Inhibitor antibody|Serine(or cysteine) proteinase inhibitor clade A(alpha 1 antiproteinase, antitrypsin) member 3 antibody|Serine(or cysteine) proteinase inhibitor clade A member 3 antibody|Serine proteinase inhibitor clade A member 3 antibody|Serpin A3 antibody|Serpin peptidase inhibitor clade A(alpha 1 antiproteinase antitrypsin) member 3 antibody|SERPINA1 antibody|SERPINA2 antibody|SERPINA3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-alpha 1 Antichymotrypsin antibody, ASA-B0071, Western blotting
WB: SMMC Cell Lysate
WB: SMMC Cell Lysate
Anti-alpha 1 Antichymotrypsin antibody, ASA-B0071, IHC(P)
Human Liver Cancer Tissue
Human Liver Cancer Tissue