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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| arachidonate 15-lipoxygenase | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human ALOX15 recombinant protein (Position: G2-P337). Human ALOX15 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat ALOX15, respectively. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ALOX15 |
Protein | Arachidonate 15-lipoxygenase |
Uniprot ID | P16050 |
Function | Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid into 12- hydroperoxyeicosatetraenoic acid/12-HPETE and 15- hydroperoxyeicosatetraenoic acid/15-HPETE. Also converts linoleic acid to 13-hydroperoxyoctadecadienoic acid. May also act on (12S)- hydroperoxyeicosatetraenoic acid/(12S)-HPETE to produce hepoxilin A3. Probably plays an important role in the immune and inflammatory responses. Through the oxygenation of membrane-bound phosphatidylethanolamine in macrophages may favor clearance of apoptotic cells during inflammation by resident macrophages and prevent an autoimmune response associated with the clearance of apoptotic cells by inflammatory monocytes. In parallel, may regulate actin polymerization which is crucial for several biological processes, including macrophage function. May also regulate macrophage function through regulation of the peroxisome proliferator activated receptor signaling pathway. Finally, it is also involved in the cellular response to IL13/interleukin-13. In addition to its role in the immune and inflammatory responses, may play a role in epithelial wound healing in the cornea maybe through production of lipoxin A4. May also play a role in endoplasmic reticulum stress response and the regulation of bone mass. |
Tissue Specificity | Detected in monocytes and eosinophils (at protein level). Expressed in airway epithelial cells. |
Sub-cellular localization | Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Lipid droplet. Note: Predominantly cytosolic; becomes enriched at membranes upon calcium binding. Translocates from the cytosol to the plasma membrane when stimulated by IL13/interleukin-13 and in macrophages binding apoptotic cells. |
Sequence Similarities | Belongs to the lipoxygenase family. |
Aliases | 15 lipoxygenase 1 antibody|15 LIPOXYGENASE RETICULOCYTE ARACHIDONATE antibody|15 LOX antibody|15 LOX 1 antibody|15-LOX antibody|15LOX 1 antibody|ALOX15 antibody|Arachidonate 15 lipoxygenase antibody|Arachidonate 15-lipoxygenase antibody|Arachidonate omega 6 lipoxygenase antibody|Arachidonate omega-6 lipoxygenase antibody|LOG15 antibody|LOX15_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ALOX15 antibody, ASA-B0069 IHC(P)
Mouse Lung Tissue
Anti-ALOX15 antibody, ASA-B0069 IHC(P)
Rat Lung Tissue
Anti-ALOX15 antibody, ASA-B0069 IHC(P)
Human Intestine Cancer Tissue
Anti-ALOX15 antibody (ASA-B0069) Western Blot
All lanes: Anti-ALOX15(ASA-B0069) at 0.5ug/ml
WB: Recombinant Human ALOX15 Protein 0.5ng
Predicted band size: 39KD
Observed band size: 39KD
All lanes: Anti-ALOX15(ASA-B0069) at 0.5ug/ml
WB: Recombinant Human ALOX15 Protein 0.5ng
Predicted band size: 39KD
Observed band size: 39KD