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More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| aldehyde dehydrogenase 3 family, member A1 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human ALDH3A1(440-453aa KVRYPPSPAKMTQH). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ALDH3A1 |
Protein | Aldehyde dehydrogenase, dimeric NADP-preferring |
Uniprot ID | P30838 |
Function | ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes. |
Tissue Specificity | High levels in stomach, esophagus and lung; low level in the liver and kidney. |
Sub-cellular localization | Cytoplasm. |
Sequence Similarities | |
Aliases | Acetaldehyde dehydrogenase 3 antibody|AHD 4 antibody|AHD C antibody|AHD4 antibody|AHDC antibody|AL3A1_HUMAN antibody|Aldehyde dehydrogenase 3 antibody|Aldehyde dehydrogenase 3 family member A1 antibody|Aldehyde dehydrogenase 3A1 antibody|Aldehyde dehydrogenase antibody|Aldehyde dehydrogenase class 3 antibody|Aldehyde dehydrogenase dimeric NADP preferring antibody|Aldehyde dehydrogenase family 3 member A1 antibody|Aldehyde dehydrogenase family 3 subfamily A1 antibody|Aldehyde dehydrogenase family 3, subfamily A, member 1 antibody|Aldehyde dehydrogenase isozyme 3 antibody|Aldehyde dehydrogenase type III antibody|ALDH 3 antibody|ALDH 3A1 antibody|ALDH III antibody|ALDH, stomach type antibody|ALDH3 A1 antibody|ALDH3 antibody|ALDH3A 1 antibody|Aldh3a1 antibody|ALDHIII antibody|dimeric NADP-preferring antibody|MGC10406 antibody|Stomach aldehyde dehydrogenase antibody|Tumor associated aldehyde dehydrogenase tumor ALDH antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ALDH3A1 antibody, ASA-B0064, Western blotting
All lanes: Anti ALDH3A1 (ASA-B0064) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Predicted bind size: 50KD
Observed bind size: 50KD
All lanes: Anti ALDH3A1 (ASA-B0064) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: A549 Whole Cell Lysate at 40ug
Predicted bind size: 50KD
Observed bind size: 50KD
Anti-ALDH3A1 antibody, ASA-B0064, IHC(P)
Human Gastric Cancer Tissue
Human Gastric Cancer Tissue