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AKT3 Antibody

ASA-B0060

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

v-akt murine thymoma viral oncogene homolog 3PolyclonalIgGRabbitHuman, Mouse, RatIHC-P, ICC, WBImmunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human AKT3(122-136aa TSQIDNIGEEEMDAS), identical to the related mouse and rat sequences.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

AKT3

Protein

RAC-gamma serine/threonine-protein kinase

Uniprot ID

Q9Y243

Function

AKT3 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase- dependent apoptosis.

Tissue Specificity

In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.

Sub-cellular localization

Nucleus.

Sequence Similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.

Aliases

Akt3 antibody|AKT3 kinase antibody|AKT3_HUMAN antibody|DKFZp434N0250 antibody|PKB gamma antibody|PKBG antibody|PRKBG antibody|Protein kinase Akt-3 antibody|Protein Kinase AKT3 antibody|Protein kinase B gamma antibody|RAC gamma antibody|RAC gamma serine/threonine protein kinase antibody|RAC-gamma serine/threonine-protein kinase antibody|RAC-PK-gamma antibody|RACPK Gamma antibody|Serine threonine protein kinase Akt 3 antibody|Serine threonine protein kinase Akt3 antibody|STK 2 antibody|STK-2 antibody|STK2 antibody|V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma antibody

Application Details

ApplicationConcentration*SpeciesValidated Using**
Western blot0.1-0.5μg/mlHuman, Mouse, RatAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)0.5-1μg/mlHuman, Rat MouseAssaySolutio's IHC/ICC Detection kit
Immunocytochemistry0.5-1μg/mlMouse Human, RatAssaySolutio's IHC/ICC Detection kit


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information

Anti-AKT3 antibody, ASA-B0060, Western blotting
Lane 1: Rat Lung Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: Human Placenta Tissue Lysate
Lane 5: A549 Cell Lysate
Lane 6: NIH3T3 Cell Lysate
 
Anti-AKT3 antibody, ASA-B0060, IHC(P)
Rat Kidney Tissue
 
Anti-AKT3 antibody, ASA-B0060, IHC(P)
Rat Lung Tissue
 

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