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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| angiotensin II receptor, type 1 | Polyclonal | IgG | Rabbit | Human, Mouse | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human AGTR1 (1-28aa MILNSSTEDGIKRIQDDCPKAGRHNYIF), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | AGTR1 |
Protein | Type-1 angiotensin II receptor |
Uniprot ID | P30556 |
Function | Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. |
Tissue Specificity | Liver, lung, adrenal and adrenocortical adenomas. |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the G-protein coupled receptor 1 family. |
Aliases | AG2S antibody|Agtr 1 antibody|Agtr1 antibody|AGTR1_HUMAN antibody|Agtr1a antibody|AGTR1B antibody|Ang II antibody| Angiotensin II receptor type 1 antibody| Angiotensin II type-1 receptor antibody|Angiotensin receptor 1 antibody| Angiotensin receptor 1B antibody|AT 1B antibody|AT 1r antibody|AT1 antibody| At1a antibody|AT1AR antibody|AT1B antibody|AT1BR antibody|AT1R antibody| AT2R1 antibody|AT2R1A antibody|AT2R1B antibody|HAT1R antibody|Type 1 angiotensin II receptor antibody|Type 1B angiotensin II receptor antibody|Type-1 angiotensin II receptor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- AGTR1 antibody, ASA-B0050, Western blotting
All lanes: Anti AGTR1 (ASA-B0050) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HEPA Whole Cell Lysate at 40ug
Predicted band size: 47KD
Observed band size: 47KD
All lanes: Anti AGTR1 (ASA-B0050) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HEPA Whole Cell Lysate at 40ug
Predicted band size: 47KD
Observed band size: 47KD
Anti- AGTR1 antibody, ASA-B0050, IHC(P)
Human Mammary Cancer Tissue
Human Mammary Cancer Tissue