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Adiponectin Antibody

ASA-B0040

$330.00

More info

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 

adiponectin, C1Q and collagen domain containingPolyclonalIgGRabbitHuman, Mouse, RatELISA, IHC-P, IHC-F, WBImmunogen affinity purified.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human ADIPOQ(224-244aa LYADNDNDSTFTGFLLYHDTN), different from the related mouse and rat sequences by one amino acid.

Properties

Form

Lyophilized

Size

100 µg/vial

Contents

Antibody is lyophilized with 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

ADIPOQ

Protein

Adiponectin

Uniprot ID

Q15848

Function

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.

Tissue Specificity

Synthesized exclusively by adipocytes and secreted into plasma.

Sub-cellular localization

Secreted.

Sequence Similarities

Contains 1 C1q domain.

Aliases

30 kDa adipocyte complement related protein antibody|30 kDa adipocyte complement-related protein antibody|ACDC antibody|ACRP 30 antibody|ACRP30 antibody|ADIPO_HUMAN antibody|Adipocyte antibody|Adipocyte C1q and collagen domain containing protein antibody|Adipocyte complement related 30 kDa protein antibody|Adipocyte complement related protein of 30 kDa antibody|Adipocyte complement-related 30 kDa protein antibody|Adiponectin antibody|AdipoQ antibody|Adipose most abundant gene transcript 1 antibody|Adipose most abundant gene transcript 1 protein antibody|Adipose specific collagen like factor antibody|ADIPQTL1 antibody|ADPN antibody|APM 1 antibody|apM-1 antibody|ApM1 antibody|C1q and collagen domain-containing protein antibody|GBP 28 antibody|GBP28 antibody|Gelatin binding protein antibody|Gelatin binding protein 28 antibody|Gelatin-binding protein antibody

Application Details

ApplicationConcentration*SpeciesValidated Using**
Western blot0.1-0.5μg/mlHuman, Rat MouseAssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)0.5-1μg/mlHuman, Mouse, Rat MouseAssaySolutio's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section)0.5-1μg/mlRat Human, MouseAssaySolutio's IHC/ICC Detection kit
ELISA0.1-0.5μg/mlHuman Human, MouseSandwich ELISA format


AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information

Anti-Adiponectin antibody, ASA-B0040, Western blotting
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Anti-Adiponectin antibody, ASA-B0040, Western blotting
E.coli derived -recombinant Human ADIPOQ, 44.4 KD(162aa tag+ M1-N244)
Lane 1: Recombinant Human ADIPOQ Protein 10ng
Lane 2: Recombinant Human ADIPOQ Protein 5ng
Lane 3: Recombinant Human ADIPOQ Protein 2.5ng
 
Anti-Adiponectin antibody, ASA-B0040, IHC(P)
Rat Kidney Tissue
 

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