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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| adiponectin, C1Q and collagen domain containing | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human adiponectin recombinant protein (Position: E19-N244). Human adiponectin shares 85% amino acid (aa) sequence identity with mouse adiponectin. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ADIPOQ |
Protein | Adiponectin |
Uniprot ID | Q15848 |
Function | Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. |
Tissue Specificity | Synthesized exclusively by adipocytes and secreted into plasma. |
Sub-cellular localization | Secreted. |
Sequence Similarities | Contains 1 C1q domain. |
Aliases | 30 kDa adipocyte complement related protein antibody|30 kDa adipocyte complement-related protein antibody|ACDC antibody|ACRP 30 antibody|ACRP30 antibody|ADIPO_HUMAN antibody|Adipocyte antibody|Adipocyte C1q and collagen domain containing protein antibody|Adipocyte complement related 30 kDa protein antibody|Adipocyte complement related protein of 30 kDa antibody|Adipocyte complement-related 30 kDa protein antibody|adipocyte-specific secretory protein antibody|Adiponectin antibody|Adiponectin precursor antibody|adiponectin, C1Q and collagen domain containing antibody|AdipoQ antibody|Adipose most abundant gene transcript 1 antibody|Adipose most abundant gene transcript 1 protein antibody|Adipose specific collagen like factor antibody|ADIPQTL1 antibody|ADPN antibody|APM 1 antibody|apM-1 antibody|ApM1 antibody|C1q and collagen domain-containing protein antibody|GBP 28 antibody|GBP28 antibody|Gelatin binding protein antibody|Gelatin binding protein 28 antibody|Gelatin-binding protein antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Adiponectin antibody, ASA-B0041, IHC(P)
Human Lung Cancer Tissue
Human Lung Cancer Tissue
Anti- Adiponectin antibody, ASA-B0041, IHC(P)
Human Mammary Cancer Tissue
Human Mammary Cancer Tissue
Anti- Adiponectin antibody, ASA-B0041, IHC(P)
Human Placenta Tissue
Human Placenta Tissue
Anti-Adiponectin (ASA-B0041), Western blotting
All lanes: Anti-Adiponectin (ASA-B0041) at 0.5ug/ml
WB: Recombinant Human Adiponectin Protein 0.5ng
Predicted band size: 35KD
Observed band size: 35KD
All lanes: Anti-Adiponectin (ASA-B0041) at 0.5ug/ml
WB: Recombinant Human Adiponectin Protein 0.5ng
Predicted band size: 35KD
Observed band size: 35KD