ASA-B0028
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Actin Antibody (monoclonal)
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Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
Monoclonal | IgG | Mouse | Chicken, Human, Mouse, Rat | IHC-F, WB | Ascites | |
Immunogen | ||||||
Synthetic actin C-terminal peptideSer-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe,attached to a Multiple Antigen Peptide(MAP) backbone. |
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Application | Concentration* | Species | Validated Using** |
Western blot | 2μg/ml | Human, chicken, mouse, rat | AssaySolutio's ECL kit |
Immunohistochemistry(Frozen Section) | 4μg/ml | Human, chicken, mouse, rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information