More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| ATP-binding cassette, sub-family B(MDR/TAP), member 6 | Polyclonal | IgG | Rabbit | Human | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human ABCB6(818-842aa YADMWQLQQGQEETSEDTKPQTMER). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ABCB6 |
Protein | ATP-binding cassette sub-family B member 6, mitochondrial |
Uniprot ID | Q9NP58 |
Function | Binds heme and porphyrins and functions in their ATP- dependent uptake into the mitochondria. Plays a crucial role in heme synthesis. |
Tissue Specificity | Widely expressed. High expression is detected in the retinal epithelium. |
Sub-cellular localization | Cell membrane. Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum. Golgi apparatus. Endosome . Note: localized to the endosome- like compartement and dendrite tips. |
Sequence Similarities | Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily. |
Aliases | ABC antibody|ABC transporter umat antibody|ABC14 antibody|Abcb6 antibody|ABCB6_HUMAN antibody|ATP binding cassette sub family B member 6, mitochondrial precursor antibody|ATP binding cassette, sub family B(MDR/TAP), member 6 antibody|ATP-binding cassette sub-family B member 6 antibody|Mitochondrial ABC transporter 3 antibody|mitochondrial antibody|Mt ABC transporter 3 antibody|Mt-ABC transporter 3 antibody|MTABC3 antibody|P glycoprotein related protein antibody|P-glycoprotein-related protein antibody|PRP antibody|Ubiquitously expressed mammalian ABC half transporter antibody|Ubiquitously-expressed mammalian ABC half transporter antibody|UMAT antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-ABCB6 antibody, ASA-B0019, Western blotting
All lanes: Anti ABCB6 (ASA-B0019) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Predicted band size: 94KD
Observed band size: 94KD
All lanes: Anti ABCB6 (ASA-B0019) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Predicted band size: 94KD
Observed band size: 94KD
Anti-ABCB6 antibody, ASA-B0019, IHC(P)
Human Mammary Cancer Tissue
Human Mammary Cancer Tissue