More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| ATP-binding cassette, sub-family B (MDR/TAP), member 4 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| E.coli-derived human ABCB4 recombinant protein (Position: A601-A720). Human ABCB4 shares 79% amino acid (aa) sequence identity with both mouse and rat ABCB4. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ABCB4 |
Protein | Multidrug resistance protein 3 |
Uniprot ID | P21439 |
Function | Mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte. |
Tissue Specificity | |
Sub-cellular localization | Cell membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. |
Aliases | ABC 21 antibody|ABC B4 antibody|ABC21 antibody|ABCB 4 antibody|Abcb4 antibody|ABCB4 protein antibody|ATP binding cassette sub family B MDR/TAP member 4 antibody|ATP binding cassette sub family B member 4 antibody|ATP-binding cassette sub-family B member 4 antibody|MDR 3 antibody|MDR2 antibody|MDR2/3 antibody|MDR3 antibody|MDR3 P glycoprotein antibody|MDR3 P gp antibody|MDR3_HUMAN antibody|Multidrug resistance protein 3 antibody|Multiple drug resistance 3 antibody|P glycoprotein 3 antibody|P-glycoprotein 3 antibody|PFIC 3 antibody|PFIC3 antibody|PGY 3 antibody|PGY3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- ABCB4 antibody, ASA-B0018, Western blotting
All lanes: Anti ABCB4 (ASA-B0018) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: 22RV1 Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Predicted band size: 142KD
Observed band size: 142KD
All lanes: Anti ABCB4 (ASA-B0018) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Lane 3: 22RV1 Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Predicted band size: 142KD
Observed band size: 142KD
Anti- ABCB4 antibody, ASA-B0018, IHC(P)
Mouse Intestine Tissue
Anti- ABCB4 antibody, ASA-B0018, IHC(P)
Rat Liver Tissue
Rat Liver Tissue