More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| ATP-binding cassette, sub-family B (MDR/TAP), member 11 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human ABCB11 (1175-1199aa KYGDNTKEIPMERVIAAAKQAQLHD), different from the related mouse and rat sequences by three amino acids. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | ABCB11 |
Protein | Bile salt export pump |
Uniprot ID | O95342 |
Function | Involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. |
Tissue Specificity | Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ. |
Sub-cellular localization | Membrane; Multi-pass membrane protein. |
Sequence Similarities | Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. |
Aliases | ABC member 16, MDR/TAP subfamily antibody|ABCB1 antibody|Abcb11 antibody| ABCBB_HUMAN antibody|ATP binding cassette sub family B (MDR/TAP) member 11 antibody|ATP binding cassette sub family B member 11 antibody|ATP-binding cassette sub-family B member 11 antibody|Bile salt export pump antibody|BRIC2 antibody|Bsep antibody|PGY4 antibody| progressive familial intrahepatic cholestasis 2 antibody|Sister of P glycoprotein antibody| Spgp antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- ABCB11 antibody, ASA-B0016, Western blotting
All lanes: Anti ABCB11 (ASA-B0016) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Predicted band size: 146KD
Observed band size: 146KD
All lanes: Anti ABCB11 (ASA-B0016) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Mouse Liver Tissue Lysate at 50ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Predicted band size: 146KD
Observed band size: 146KD
Anti- ABCB11 antibody, ASA-B0016, IHC(P)
Mouse Liver Tissue
Mouse Liver Tissue
Anti- ABCB11 antibody, ASA-B0016, IHC(P)
Rat Liver Tissue
Rat Liver Tissue