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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| 5-hydroxytryptamine(serotonin) receptor 3A, ionotropic | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence in the middle region of human5HT3A receptor(140-154aa RHQGEVQNYKPLQVV). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | HTR3A |
Protein | 5-hydroxytryptamine receptor 3A(5-HT3-A/5-HT3A) |
Uniprot ID | P46098 |
Function | This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel. |
Tissue Specificity | Expressed in cerebral cortex, amygdala, hippocampus, and testis. Detected in monocytes of the spleen and tonsil, in small and large intestine, uterus, prostate, ovary and placenta. |
Sub-cellular localization | Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. |
Sequence Similarities | |
Aliases | 5 HT 3 antibody|5 HT3 receptor antibody|5 HT3A antibody|5 HT3A receptor antibody|5 HT3R antibody|5 HT3RA antibody|5 hydroxytryptamine(serotonin) receptor 3A antibody|5-HT-3 antibody|5-HT3-A antibody|5-HT3A antibody|5-HT3R antibody|5-hydroxytryptamine receptor 3 antibody|5-hydroxytryptamine receptor 3A antibody|5HT 3 antibody|5HT 3A antibody|5HT receptor antibody|5HT3 serotonin receptor antibody|5HT3A_HUMAN antibody|5HT3R antibody|HTR3 antibody|Htr3a antibody|Serotonin gated ion channel receptor antibody|Serotonin receptor 3A antibody|Serotonin-gated ion channel receptor 3a antibody|Serotonin-gated ion channel receptor antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-5HT3A receptor antibody, ASA-B0009, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SW620 Cell Lysate
Lane 1: U87 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SW620 Cell Lysate