Data sheet
| Assay Range | 7.8 - 500 pg/ml |
More info
Sensitivity
5 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human VWC2 standard: 1 ng/vial - 2.
2. One 96-well plate precoated with anti-human VWC2 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human VWC2 Ab: 1 vial, dilution 1:250.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
VWC2 (von Willebrand factor C domain containing 2), also known as brorin or brain-specific chordin-like protein, is a 325 amino acid secreted protein that contains two VWFC domains and belongs to the Chordin family. VWC2 expression increases during brain development, with strong levels found in neurons of the diencephalon and medulla oblongata. Acting as a bone morphogenetic protein (BMP) antagonist, VWC2 inhibits the activities of BMP-2 and BMP-6 during neurogenesis in adult and developing neural tissues, and may play a role in neural development and function. VWC2 may also have a role in promoting cell adhesion.
