Data sheet
| Assay Range | 62.5 - 4000 pg/ml |
More info
Sensitivity
60 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human VTN standard: 10 ng/vial - 2.
2. One 96-well plate precoated with anti-human VTN Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human VTN Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Vitronectin also known as VTN is a protein that is a member of the pexin family. Vitronectin is an abundant glycoprotein found in serum and the extracellular matrix . Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Vitronectin has been speculated to be involved in hemostasis and tumor malignancy.
