More info
Assay Range | 156 - 10,000 pg/mL |
Sensitivity | 2.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human TIMP-3 standard: 2 vials
2. One 96-well plate precoated with anti- Human TIMP-3 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
The tissue inhibitor of metalloproteinase 3 (TIMP-3), also known as protein MIG-5, is a member of the tissue inhibitor of metalloproteinase (TIMP) family in which four proteins TIMP-1, TIMP-2, TIMP-3 and TIMP-4 have been identified so far. TIMPs are natural inhibitors of the matrix metalloproteinases (MMPs) which are involved in degradation of the extracellular matrix and exhibit diverse functions in development, tissue remodeling, wound healing and tumor metastasis. TIMPs block access of substrates to the MMP catalytic site by forming 1:1 non-covalent complexes with MMPs. Basically, TIMPs are highly specific for MMPs but may not be able to digest some particular MMP such as MMP14. TIMP-3 is expressed by many different cells and is the only known member of the TIMP family that exhibits inhibitory activity on ADAMs such as TACE. It is reported that point mutations in the C-terminal domain of TIMP-3 are correlated with Sorsby's fundus dystrophy.