Data sheet
| Assay Range | 78.125 - 5000 pg/ml |
More info
Sensitivity
70 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human STK4 standard: 10 ng/vial - 2.
2. One 96-well plate precoated with anti-human STK4 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human STK4 Ab: 1 vial, dilution 1:700.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Serine/threonine-protein kinase 4, also known as Mammalian STE20-like protein kinase 1, STE20-like kinase MST1, is a cytoplasm and nucleus protein which belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. STK4 acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. STK4 is expressed in prostate cancer and levels increase from the normal to the malignant state. It is a key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis.
