Human SerpinF2 ELISA Kit
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  • Human SerpinF2 ELISA Kit

Human SerpinF2 ELISA Kit

Catalog # NS-E10131

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$625.00
Quantity

For research use only. Not for diagnostic or therapeutic use, or for human or veterinary use. Sold only to research institutions and qualified laboratory professionals. Not for sale to individuals for personal use.
Sensitivity
60 pg/mL
 
Size
96T
 
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
 
Assay Principle
Sandwich ELISA
 
Sample volume
100 µL final volume, dilution factor varies on samples.
 
Detection Method
Chromogenic
 
 
 
 
Kit Components
 
 
 
1. Recombinant human SerpinF2 standard: 10 ng/vial - 2.
 
 
2. One 96-well plate precoated with anti-human SerpinF2 Ab
 
 
3. Sample diluent buffer: 12 mL x 1
 
 
4. HRP conjugated anti-Human SerpinF2 Ab: 1 vial, dilution 1:1000.
 
 
5. Detection Antibody diluent buffer: 12 mL x 1
 
 
6. 20 x Wash Solution: 25 ml x 1.
 
 
7. TMB color developing agent A: 6 ml.
 
 
8. TMB color developing agent B: 6 ml.
 
 
9. TMB stop solution: 6ml.

 

Background

 

SerpinF2 (or α2-antiplasmin or plasmin inhibitor), is a serine protease inhibitor (serpin) responsible for inactivating plasmin, an important enzyme that participates in fibrinolysis and degradation of various other proteins. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. It is expressed by the liver and secreted in plasma. Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID).

Standard Curve

Data sheet

Assay Range
62.5 - 4000 pg/ml
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