Data sheet
| Assay Range | 62.5 - 4000 pg/ml |
More info
Sensitivity
60 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human SerpinF2 standard: 10 ng/vial - 2.
2. One 96-well plate precoated with anti-human SerpinF2 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human SerpinF2 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
SerpinF2 (or α2-antiplasmin or plasmin inhibitor), is a serine protease inhibitor (serpin) responsible for inactivating plasmin, an important enzyme that participates in fibrinolysis and degradation of various other proteins. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. It is expressed by the liver and secreted in plasma. Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID).
