Data sheet
| Assay Range | 7.8 - 500 pg/ml |
More info
Sensitivity
5 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human PRLR standard: 1 ng/vial - 2.
2. One 96-well plate precoated with anti-human PRLR Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human PRLR Ab: 1 vial, dilution 1:2000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Prolactin receptor (PRLR) is a receptor for the anterior pituitary hormone, prolactin, and belongs to the type I cytokine receptor family. Prolactin receptors have been identified in number of cells and tissues including the mammary gland, organs of the reproductive system, central nervous system, pituitary, adrenal cortex, skin, bone, lung, heart, liver, pancreas, GI tract, kidney, lymphoid tissue and spermatozoa. PRLR is located in the cell membrane, but also present intracellularly at various compartments. PRLR mediates prolactin signaling and triggers intracellular responses that participate in diverse biological functions including, mammary gland development (proliferation and differentiation).
