More info
Assay Range | 3.12--2000 pg/mL |
Sensitivity | 3.0 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Sample Type | Cell lysates |
Detection Method | Chromogenic |
Kit Components
1. Human Phospho-MKK6 (S207/T211) standard: 2 vials
2. One 96-well plate coated with Phospho-MKK6 (S207/T211) Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 1 vial
5. Streptavidin-HRP: 1 vial
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. Chromogenic solution A: 6 mlx1
9. Chromogenic solution B: 6 mlx1
10. Stop solution: 6 mL x1
11. Washing solution (20x): 25 mL x1
Background
MAPKK6, also known as MAPK/ERK kinase 6, is a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MKK6 plays an important role in phosphorylating and activating p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this protein is involved in many cellular processes such as stress-induced cell cycle arrest, transcription activation and apoptosis.