More info
Assay Range | 156 -10,000 pg/mL |
Sensitivity | 10 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human phospho-Erythropoietin R (Y426) standard: 2 vials
2. One 96-well plate coated Human phospho-Erythropoietin R (Y426) Ab
3. Diluent buffer 1: 12 ml
4. Diluent buffer 2: 20 ml
5.Detection antibody: 12 ml
6.Chromogenic Solution A : 11 ml.
7. Chromogenic Solution B : 11 ml.
8. Stop solution: 11 mL x1
9.. Washing solution (20x): 25 mL x1
Background
The erythropoietin receptor (EpoR), encoded by the EPOR gene in humans, is a member of the cytokine receptor family. EpoR pre-exists as dimmers, which upon binding of its ligand erythropoietin (Epo), changes its homodimerized state. These conformational changes result in the autophosphorylation of Jak2 kinases that are associated with the receptor The most well-established function of EpoR is to rescue erythroid progenitors from apoptosis.