Data sheet
| Assay Range | 62.5 - 4000 pg/ml |
More info
Sensitivity
60 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human PDGFRb standard: 4 ng/vial - 2.
2. One 96-well plate precoated with anti-human PDGFRb Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human PDGFRb Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Beta-type platelet-derived growth factor receptor, also known as CD140b, PDGFRb, PDGF-R-beta, PDGF receptor-β, is a member of the protein kinase superfamily and CSF-1 / PDGF receptor subfamily. In epithelial tumors, PDGFRb is mainly expressed by stromal cells of mesenchymal origin. Tumor cells may also acquire PDGFRb expression following epithelial-to-mesenchymal transition (EMT), which occurs during metastasis formation.
