Data sheet
| Assay Range | 62.5 - 4000 pg/ml |
More info
Sensitivity
60 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human PBK standard: 4 ng/vial - 2.
2. One 96-well plate precoated with anti-human PBK Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human PBK Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
PBK is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. It is phosphorylated during mitosis and may be involved in the activation of lymphoid cells and support testicular functions. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.
