Data sheet
| Assay Range | 15.6 - 1000 pg/ml |
More info
Sensitivity
10 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human LAMP-1 standard: 2 ng/vial - 2.
2. One 96-well plate precoated with anti-human LAMP-1 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human LAMP-1 Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Lysosomal-associated membrane protein 1 (LAMP1) also known asCD107a (Cluster of Differentiation 107a), is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis.CD107a has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells.
