Data sheet
| Assay Range | 31.25 - 2000 pg/ml |
More info
Sensitivity
30 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human IL20RA standard: 4 ng/vial - 2.
2. One 96-well plate precoated with anti-human IL20RA Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human IL20RA Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
IL-20, an IL-10-like cytokine, signals through a heterodimeric complex consisting of IL-20 R alpha and a second component IL-20 R beta. Both subunits are class II cytokine receptors containing two fibronectin type III domains and a tissue factor-like domain in the extracellular region. IL-20 R alpha and IL-20 R beta are expressed in a variety of tissues. The expression of both IL20RA and IL20RB is found to be upregulated in psoriatic skin lesions on keratinocytes.
