Data sheet
| Assay Range | 3.9 - 250 pg/ml |
More info
Sensitivity
3 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human IL 12B standard: 250pg/vial - 2.
2. One 96-well plate precoated with anti-human IL 12B Ab
3. Sample diluent buffer: 12 mL x 1
4. Biotin-conjugated anti-Human IL-12B Ab: 1 vial.
5. Detection Antibody diluent buffer: 12 mL x 2
6. Streptavidin-HRP: 1 vial, 1:200 dilution.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by IL12B, and a 35 kD subunit encoded by IL12A. Interleukin 12B is expressed acts on T and natural killer cells, and has a broad array of biological activities. IL-12B that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
