Data sheet
| Assay Range | 78.125 - 5000 pg/ml |
More info
Sensitivity
50 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human IL10RB standard: 10 ng/vial - 2.
2. One 96-well plate precoated with anti-human IL10RB Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human IL10RB Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Interleukin 10 receptor, beta subunit is a subunit for the interleukin-10 receptor. IL10RB belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complexes. Coexpression of IL10RB and IL10RA proteins has been shown to be required for IL10-induced signal transduction.
