Data sheet
| Assay Range | 156 - 10000 pg/ml |
More info
Sensitivity
150 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human IGF1R standard: 10 ng/vial - 2.
2. One 96-well plate precoated with anti-human IGF1R Ab
3. Sample diluent buffer: 12 mL x 1
4. Biotin-conjugated anti-Human IGF1R Ab: 1 vial.
5. Detection Antibody diluent buffer: 12 mL x 2
6. Streptavidin-HRP: 1 vial, 1:200 dilution.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Insulin-like growth factor 1 receptor, also known as Insulin-like growth factor I receptor , IGF1R, and CD221, is a single pass type I membrane protein which belongs to the protein kinase superfamily, Tyr protein kinase family and Insulin receptor subfamily. It is activated by a hormone called insulin-like growth factor 1 (IGF-1) and by a related hormone called IGF-2. IGF1R mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin and it also plays an important role in growth and continues to have anabolic effects in adults - meaning that it can induce hypertrophy of skeletal muscle and other target tissues.
