Data sheet
| Assay Range | 31.25 - 2000 pg/ml |
More info
Sensitivity
10 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human Fetuin-A standard: 2 ng/vial - 2.
2. One 96-well plate precoated with anti-human Fetuin-A Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human Fetuin-A Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Alpha2-HS glycoprotein (AHSG), also known as fetuin-A, is an abundant plasma protein that predicts vascular risk in a variety of clinical settings. It displays high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification.
