Data sheet
| Assay Range | 39 - 2500 pg/ml |
More info
Sensitivity
30 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human Ephrin-A1 standard: 10 ng/vial - 2.
2. One 96-well plate precoated with anti-human Ephrin-A1 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human Ephrin-A1 Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
EphrinA1, also known as B61 and LERK1, is a member of the EphrinA family of GPIanchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin- ligands are structurally related to the extracellular domains of the transmembrane
EphrinB ligands. Ephrin interactions are widely involved in the regulation of cell migration,
tissue morphogenesis, and cancer progression.
