Data sheet
| Assay Range | 62.5 - 4000 pg/ml |
More info
Sensitivity
50 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human DKK3 standard: 4 ng/vial - 2.
2. One 96-well plate precoated with anti-human DKK3 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human DKK3 Ab: 1 vial, dilution 1:100.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
DKK3 is a protein that belongs to the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. DKK3 is highest expressed in brain, eye and heart. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene.
