Data sheet
| Assay Range | 31.25 - 2000 pg/ml |
More info
Sensitivity
30 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CEACAM5 standard: 2 ng/vial - 2.
2. One 96-well plate precoated with anti-human CEACAM5 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CEACAM5 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Carcinoembryonic antigen (CEA, CEACAM5, and CD66e) belongs to the CEA subgroup of 7 closely related molecules (CD66a-e, CGM2, CGM7) within the CEA Gene Family, which is part of the immonoglobulin superfamily of glycoproteins. It has been found to be associated with various types of cancers, particularly colorectal carcinoma, and developed to be a molecular target for cancer diagnosis and therapy.
