Data sheet
| Assay Range | 3.125 - 200 pg/ml |
More info
Sensitivity
2 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CD83 standard: 1 ng/vial - 2.
2. One 96-well plate precoated with anti-human CD83 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CD83 Ab: 1 vial, dilution 1:400.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
CD83 is an immunoglobulin (Ig) superfamily which belongs to single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The encoded protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. It has been widely used as a marker for mature DCs..
