Data sheet
| Assay Range | 23.44 - 1500 pg/ml |
More info
Sensitivity
20 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CD55 standard: 3ng/vial - 2.
2. One 96-well plate precoated with anti-human CD55 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CD55 Ab: 1 vial, dilution 1:2000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
CD55, also called decay accelerating factor (DAF), is a GPI-anchored single chain glycoprotein. Binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. CD55 functions to prevent the assembly of C3 convertase or to accelerate the disassembly of preformed convertase, which blocks formation of the membrane attack complex. CD55 is expressed on cells in contact with serum,including hematopoietic and many non-hematopoietic cells.
