Data sheet
| Assay Range | 93.8 - 6000 pg/ml |
More info
Sensitivity
93.8 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CD38 standard: 6 ng/vial - 2.
2. One 96-well plate precoated with anti-human CD38 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CD38 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
CD38 (cluster of differentiation 38), also known as cyclic ADP ribose hydrolaseis a glycoprotein found on the surface of many immune cells (white blood cells), including CD4+, CD8+, B and natural killer cells. CD38 also functions in cell adhesion, signal transduction and calcium signaling. The loss of CD38 function is associated with impaired immune responses, metabolic disturbances, and behavioral modifications.
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