Data sheet
| Assay Range | 156 - 10000 pg/ml |
More info
Sensitivity
156 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CD31 standard: 10 ng/vial - 2.
2. One 96-well plate precoated with anti-human CD31 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-HumanCD31 Ab: 1 vial, dilution 1:250.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
CD31, also known as platelet endothelial cell adhesion molecule (PECAM-1), is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrinactivation. CD31 is expressed in platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. CD-31 is also expressed in certain tumors, including epithelioid hemangioendothelioma, epithelioid sarcoma-like hemangioendothelioma, othervascular tumors, histiocytic malignancies, and plasmacytomas. It is rarely found in some sarcomas, such as Kaposi's sarcoma, and carcinomas.
