> >>Human CD146 ELISA Kit ( MCAM/ MUC18 )
View larger

Human CD146 ELISA Kit ( MCAM/ MUC18 )

NS-E10025

$599.00

Data sheet

Assay Range 31.25 - 2000 pg/ml

More info

Sensitivity
10 pg/mL
 
Size
96T
 
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
 
Assay Principle
Sandwich ELISA
 
Sample volume
100 µL final volume, dilution factor varies on samples.
 
Detection Method
Chromogenic
 
 
 
 
Kit Components
 
 
 
1. Recombinant human CD146 standard: 2 ng/vial - 2.
 
 
2. One 96-well plate precoated with anti-human CD146 Ab
 
 
3. Sample diluent buffer: 12 mL x 1
 
 
4. HRP conjugated anti-Human CD146 Ab: 1 vial, dilution 1:1000.
 
 
5. Detection Antibody diluent buffer: 12 mL x 1
 
 
6. 20 x Wash Solution: 25 ml x 1.
 
 
7. TMB color developing agent A: 6 ml.
 
 
8. TMB color developing agent B: 6 ml.
 
 
9. TMB stop solution: 6ml.

 

Background

 

CD146 (cluster of differentiation 146) also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. CD146 is highly expressed by cells that are components of the blood vessel wall, including vascular endothelial cells, smooth muscle cells and pericytes. Its function is still poorly understood, but evidence points to it being part of the endothelial junction associated with the actin cytoskeleton. CD146 has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency, mesenchymal stem cells with greater differentiation potential express higher levels of CD146 on the cell surface.


Standard Curve

Download

Protocol

Download (0)

© 2024 Novateinbio.com