Data sheet
| Assay Range | 7.8 - 500 pg/ml |
More info
Sensitivity
5pg/mL
Size
96T
Assay Principle
Sandwich ELISA
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Sample Volume
100 µL final volume, dilution factor varies on samples
Detection Method
Chromogenic
Kit Components
1. Recombinant human CA12 standard: 2ng/vial - 2.
2. One 96-well plate precoated with anti-human CA12 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CA12 Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. Assay diluent buffer: 12 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene.
