Data sheet
| Assay Range | 7.8 - 500 pg/ml |
| Sensitivity | 5 pg/ml |
| Size | 96T |
| Storage | Store at 2 - 8⁰C. Keep Standard and detection antibody at -20⁰C. |
| Expiration | Six months at 2 - 8 ⁰C. |
| Application | For quantitative determination of Human BID. |
| Assay principle | This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). |
| Sample type | Serum, plasma, body fluids, tissue lysates. |
More info
Sample Volume
100 µL final volume, dilution factor varies on samples
Detection Method
Chromogenic
Kit Components
1. Recombinant BID standard: 1ng/vial - 2.
2. One 96-well plate precoated with anti-BID antibody.
3. Sample diluent buffer: 12 ml
4. HRP conjugated anti-BID antibody: 1 vial, dilution 1:4000.
5. Detection Antibody diluention buffer: 12ml.
6. Assay diluent buffer: 12 ml.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
The BID (BH3 interacting-domain death agonist) is a pro-apoptoticmember of the Bcl-2 protein family. It response to apoptotic signaling. BID interacts with another Bcl-2 family protein, Bax, leading to the insertion of Bax into organelle membranes, primarily the outermitochondrial membrane. The expression of BID is upregulated by the tumor suppressor p53, and BID has been shown to be involved in p53-mediated apoptosis.
